Part:BBa_K4430010:Design
GFP-TFP
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2201
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2169
Illegal AgeI site found at 1824
Illegal AgeI site found at 2262 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 641
Illegal BsaI.rc site found at 1263
Design Notes
TFP is a receptor binding protein (RBP) specifically recognizing E. coli strains. GFP emits green fluorescence under the confocal laser scanning microscope. Using fusion protein GFP-TFP, we can confirm whether TFP binds strongly to the cell surfaces of E.coli. Commonly, when interdomain movement and association of fusion protein is desired, flexible linkers are a good choice. Flexible linkers are usually passive; they serve by retaining distance between functional protein motifs. The length of flexible linkers is the key property that determines the appropriate folding in fusion proteins, if not achieved, leads to the formation of non-functional aggregates. The combination of non-polar Gly residues with the polar Ser or Thr is routinely used for synthesizing the flexible linkers. The flexible linkers are proven helpful for stabilizing such types of neighbors by facilitating the covalent bonding among them. So the flexible linker GGSG was used as fusion protein linker in GFP-TFP.
Source
GFP is from BBa_K404316. Protein linker is from BBa_K4430004. TFP is from BBa_K4430009.